Sains Malaysiana 52(9)(2023): 2599-2612

http://doi.org/10.17576/jsm-2023-5209-10

Volatile Compounds Detected by SPME-GC-MS in Fish Viscera Extracts with Attractant Activity against House Fly Musca domesticafrom Chemometric FTIR Fingerprints

(Sebatian Meruap Dikesan oleh SPME-GC-MS dalam Ekstrak Visera Ikan dengan Aktiviti Bahan Penarik terhadap Lalat Rumah Musca domestica daripada Cap Jari FTIR Kimometriks)

RAHMATIA GARWAN^1,4,5, HANIFAH NURYANI LIOE^1,*, TATI NURHAYATI², NANCY DEWI YULIANA¹, SUPRIYONO ³ & HARSI DEWANTARI KUSUMANINGRUM¹

¹Department of Food Science and Technology, Faculty of Agricultural Engineering and Technology, IPB University, Bogor 16680, Indonesia

 ²Department of Fishery Products Technology, Faculty of Fisheries and Marine Sciences, IPB University, Bogor 16680, Indonesia

³Department of Animal Disease Science and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Bogor 16680, Indonesia

⁴Food Agency of the Local Government of North Moluccas Province, Indonesia

⁵Faculty of Agriculture, Muhammadiyah University of North Moluccas, Indonesia

Received: 23 April 2023/Accepted: 17 August 2023

Abstract

Fish viscera has been locally applied as fly attractant in North Moluccas. The objective of this study was to evaluate the efficacy of attractant from fish viscera extracts and to determine the indispensable active extracts with chemometric FTIR fingerprints, as well as to characterize volatile compounds of the extracts. Attractant activity against house fly Musca domesctica and FTIR spectra were analyzed for the ethanol, acetone and hexane extracts of stomach, intestines, liver, and combination of the viscera from skipjack tuna stored for 1-2 days at room temperature. SPME-GC-MS analysis was applied for active extracts to determine volatile compounds. OPLS analysis result showed that FTIR spectra were correlated with the attractant activity (R² 0.974 and Q² 0.89), and compounds with functional groups S-H, N-H, O-H, and C-O have the strong correlation. Ethanol and acetone extracts of mixed viscera from 2 days storage are the active extracts. The volatile compounds in the extract through the SPME-GC-MS analysis belong to ketone, amine, phenol, heterocyclic, aromatic aldehyde, fatty acid, fatty acid ester and sulfur compounds are reported.

Keywords: Attractant activity; fish viscera extracts; Musca domestica; chemometric; FTIR fingerprint; SPME-GC-MS

Abstrak

Visera ikan telah digunakan secara tempatan sebagai bahan penarik lalat di Maluku Utara. Objektif kajian ini adalah untuk menilai keberkesanan bahan penarik daripada ekstrak visera ikan dan menentukan ekstrak aktif yang sangat diperlukan dengan cap jari FTIR kimometriks selain mencirikan sebatian meruap bagi ekstrak tersebut. Aktiviti bahan penarik terhadap lalat rumah Musca domesctica dan spektrum FTIR dianalisis untuk ekstrak etanol, aseton dan heksana perut, usus, hati dan gabungan visera daripada tuna cakalang yang disimpan selama 1-2 hari pada suhu bilik. Analisis SPME-GC-MS digunakan untuk ekstrak aktif dalam menentukan sebatian meruap. Keputusan analisis OPLS menunjukkan bahawa spektrum FTIR berkorelasi dengan aktiviti bahan penarik (R² 0.974 dan Q² 0.89) serta sebatian kumpulan berfungsi S-H, N-H, O-H dan C-O mempunyai korelasi yang kuat. Ekstrak etanol dan aseton daripada gabungan visera daripada simpanan 2 hari adalah merupakan ekstrak aktif. Sebatian meruap dalam ekstrak melalui analisis SPME-GC-MS dilaporkan tergolong kepada keton, amina, fenol, heterosiklik, aldehid aromatik, asid lemak, ester asid lemak dan sebatian sulfur.

Kata kunci: Aktiviti bahan penarik; cap jari FTIR; ekstrak visera ikan; kimometriks; Musca domestica; SPME-GC-MS

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*Corresponding author; email: hanifahlioe@apps.ipb.ac.id